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Implications draw on the history of transformative information systems from the past 

High resolution mobility-based ion separations in Structures for Lossless Ion Manipulations (SLIM) have been useful for ion mobility separations for a variety of molecular classes in the gas phase. Here, we present multi-pass SLIM separations for gas-phase proteins in their near-native state exhibiting charge state dependent arrival time distributions using carbonic anhydrase (29 kDa), alcohol dehydrogenase (148 kDa), and apo-transferrin (79 kDa). For the selected charge states of each protein species, we investigate the conformational space using molecular dynamic simulations and calculated the collision cross section (CCS) values using IMoS. The measured CCS values obtained from the SLIM arrival time distributions (ATDs) agreed within ~6% difference when compared to the calculated CCS values. The experimental CCS values were obtained from calibration curves for the arrival times of Agilent Tune Mix ions. For multi-pass separations, the ATDs were converted to CCS values by deconvoluting the multi-pass arrival times into accurate single-pass values amenable to the single-pass calibration curves. Mass spectra of carbonic anhydrase (CA) showed three different charge states (z = 9+ to 11+). Their corresponding mobility peaks were baseline-separated using 8-m single-pass separations. Single-pass analysis of alcohol dehydrogenase (ADH) exhibit three predominant charge states (z = 23+ to 25+) with mobility overlap between adjacent charge states. The mobility peak resolution for ADH improved with multi-pass separations (up to 24-m path length). In addition, CCS distributions obtained for charge states z = 16+ to 18+ of apo-transferrin reveal a transition from a compact unimodal form (z = 18+ and 19+) to broader multi-modal CCS distributions for z = 16+. For apo-transferrin, 40-m multi-pass separations were performed allowing for complete isolation of the selected mobility range corresponding to z = 17+ leading to selective isolation of a narrow arrival time window. The extended mobility separations provided minimal alterations to the structure of the proteins, and the experimentally derived CCS values showed minimal change as a function of separation time or number of passes. Mobility-based ion separations for native-like proteins, using SLIM, open opportunities for native-IMS applications as well as other manipulations enabled by SLIM like mobility selective isolation and collection. 

Microbial mats are stratified communities often dominated by unicellular and filamentous phototrophs within an exopolymer matrix. It is challenging to quantify the dynamic responses of community members in situ as they experience steep gradients and rapid fluctuations of light. To address this, we developed a binary consortium using two representative isolates from hot spring mats: the unicellular oxygenic phototrophic cyanobacteriumSynechococcusOS-B′ (Syn OS-B′) and the filamentous anoxygenic phototrophChloroflexusMS-CIW-1 (Chfl MS-1). We quantified the motility of individual cells and entire colonies and demonstrated that Chfl MS-1 formed bundles of filaments that moved in all directions with no directional bias to light. Syn OS-B′ was slightly less motile but exhibited positive phototaxis. This binary consortium displayed cooperative behavior by moving further than either species alone and formed ordered arrays where both species aligned with the light source. No cooperative motility was observed when a nonmotilepilBmutant of Syn OS-B′ was used instead of Syn OS-B′. The binary consortium also produced more adherent biofilm than individual species, consistent with the close interspecies association revealed by electron microscopy. We propose that cyanobacteria and Chloroflexota cooperate in forming natural microbial mats by colonizing new niches and building robust biofilms. 

We examine deformed crystalline bedrock in the upper parts of the active San Andreas and ancient San Gabriel Faults, southern California, to 1) determine the nature and origin of micro-scale composition and geochemistry of fault-related rocks, 2) constrain the extent of fluid-rock interactions, and 3) determine the interactions between alteration, mineralization, and deformation. We used drill cores from a 470 m long inclined borehole through the steep-dipping San Gabriel Fault and from seven inclined northeast-plunging boreholes across the San Andreas Fault zone to 150 m deep to show that narrow fault cores 10 cm to 5 m wide lie within 100s m wide damage zones. Petrographic, mineralogic, whole-rock geochemical analyses and synchrotron-based X-ray fluorescence mapping of drill core and thin sections of rocks from the damage zone and narrow principal slip surfaces reveal evidence for the development of early fracture networks, with iron and other transition element mineralization and alteration along the fractures. Alteration includes clay $$\pm$$ chlorite development, carbonate, and zeolite mineralization in matrix and fractures and the mobility of trace and transition elements. Carbonate-zeolite mineralization filled fractures and are associated with element mobility through the crystalline rocks. Textural evidence for repeated shearing, alteration, vein formation, brittle deformation, fault slip, pressure solution, and faulted rock re-lithification indicates significant hydrothermal alteration occurred during shallow-level deformation in the fault zones. The rock assemblages show that hydrothermal conditions in active faults develop at very shallow levels where seismic energy, heat, and fluids are focused.